Tip Head Ultrasonic Homogenizer Assisted Liquid Ultrasonic Homogenizer Virus Cells / Bacteria Extraction

What's the theory of ultrasonic Cell Pulverizer?

Ultrasonic is an elastic mechanical wave in a matter medium, which is a form of wave, so it can be used to detect physiological and pathological information of the human body, and to diagnose ultrasound. At the same time, it is a form of energy. When a certain dose of ultrasound is transmitted in the living body, the interaction between the two can cause the function and structure of the organism to change, that is, the ultrasonic biological effect. The effects of ultrasound on cells are mainly thermal effects, cavitation effects and mechanical effects. The thermal effect is that when the ultrasound propagates through the medium, the friction hinders the molecular vibration caused by the ultrasound, converting part of the energy into local high heat (42-43 ° C), because the critical lethal temperature of normal tissue is 45.7 ° C, and the tumor tissue ratio Normal tissue sensitivity is high, so at this temperature, the metabolism of tumor cells is impaired, DNA, RNA, and protein synthesis are affected, thereby killing cancer cells and normal tissues are not affected. The cavitation effect is that under the irradiation of ultrasound, vacuoles are formed in the living body, and mechanical shear pressure and turbulence are generated along with the vibration of the vacuole and its violent explosion, which causes the tumor to hemorrhage and the tissue to collapse and cause necrosis. In addition, when the cavitation bubble bursts, it generates instantaneous high temperature (about 5000 ° C) and high pressure (up to 500 × 104 Pa), which can thermally dissociate the water vapor to produce .OH radicals and .H atoms, which are composed of .OH radicals and .H. Atomic induced redox reactions can lead to polymer degradation, enzyme inactivation, lipid peroxidation, and cell killing. The mechanical effect is the primary effect of ultrasound. During the propagation process, the medium particles alternately compress and stretch to form a pressure change, causing damage to the cell structure. The intensity of killing is closely related to the frequency and intensity of ultrasound.

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Application:

Ultrasonic Cell Pulverizer High Power 20Khz for Extraction of Polygonatum Polysaccharide used in a variety of animal and plant cells, virus cells, bacteria, dental spores and tissue ultrasonic extraction method using ultrasonic waves, its powerful cavitation effect and many secondary effects such as: mechanical vibration, crushing emulsification, diffusion, etc. can break the cell wall release Substance, commonly used in the breakdown of various animal and plant cells, viral cells, bacteria, spores and tissues. In this study, ultrasonic polysaccharide cell pulverizer was used to extract Polygonatum polysaccharide. The obtained polysaccharide was lighter in color, less impurities and higher purity than water decoction and dilute alkali extraction. It is an effective method for extracting Polygonatum polysaccharide.

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